SCAR-marking application for genotyping of the Сf-9 locus in new tomato breeding accessions

UDC 632.4.01:577.21

Pyrsikov A.S., Milyukova N.A.

Tomato (Solanum lycopersicum) is one of the most important vegetable crops. The injury by various diseases leads to a decrease in yield, deterioration of products, a decrease in the amount of useful substances and the storability that are the main reasons of the serious financial losses. One of the most dangerous diseases of tomato is leaf mould, the causative agent of which is the fungus Cladosporium fulvum. The main method of disease control is the breeding and growing of resistant tomato cultivars and hybrids. Traditional breeding is a long process andmolecular markers are effective at various stages of the breeding process. Tomato resistance to leaf mould is inherited as a completely dominant trait. The genetic mechanism of leaf mould resistance is controlled by 24 genes, among which the Cf-9 gene is one of the main ones. The Cf-9 locus has been integrated into cultivated tomato from its wilde relative Solanum pimpinellifolium. Molecular labeling methods aimed at identifying alleles of the Cf-9 gene are increasingly being used in breeding programs. The system of SCAR-marking used in current investigation make possible to detect resistant and susceptible to leaf mold genotypes. To confirm the efficiency of the method, tests in place were carried out against an infectious background. It was found that the field score is fully congruent with the results of genotyping. These results shows that the used system of SCAR marking of Cf-9 gene alleles can be successfully used in MAS and breeding of tomato new varieties and hybrids resistant to C. fulvum.

Key words: Cf-9 locus, Cladosporium fulvum, MAS, SCAR-marking

Pyrsikov A.S., Cand. Sci. (Agr.), senior research fellow, Molecular Biology Department, The Russian State Center for Animal Feed and Drug Standardization and Quality; research fellow of Laboratory of Marker and Genomic Plant Breeding, FSBSI All-Russia Research Institute of Agricultural Biotechnology (ARRIAB).E-mail:

Milyukova N.A., Cand. Sci. (Biol.), senior research fellow of Laboratory of Marker and Genomic Plant Breeding, FSBSI All-Russia Research Institute of Agricultural Biotechnology (ARRIAB). E–mail:

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For citing: Pyrsikov A.S., Milyukova N.A. SCAR-marking application for genotyping of the Сf-9 locus in new tomato breeding accessions. Potato and vegetables. 2024. No2. Pp.  52-56. (In Russ.).

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