UDC 631.527.22
https://doi.org/10.25630/PAV.2024.92.21.002
Solovyeva J.A., Monakhos S.G.
Doubled haploids production technologies in acceleration of the breeding process have become widespread all over the world. The use of DH technologies promotes a significant reduction (up to 6–12 times) of homozygous lines production. These lines are used in breeding as parent components in the F1-hybrid creation of vegetable crops. In this study, the effect of the explants cultivation light mode during heat treatment and the use of casein hydrolysate in the nutrient medium on the plant genus Cucurbita L. representatives (C. pepo L., C. maxima L., C. moschata L.) gynogenesis induction was studied. Isolated ovules were cultured on a solid CBM nutrient medium supplemented with 30 g/l sucrose and 7 g/l agar (pH 5.8). Explants were exposed to temperature treatment (32 °C for 4 days), and then cultured under conditions of a 16-hour photoperiod, 24 °C and high humidity. As a result of the study, the species peculiarity of explant reaction to changes in the cultivation light regime during the heat treatment was revealed. C. pepo explants showed a significant increase in the degree of responsiveness to gynogenesis induction when using a 16-hour photoperiod. The ovules of C. maxima developed better in conditions of complete absence of illumination. No significant differences were found in C. moschata explants when using different lighting modes. Also, based on the results obtained, the positive effect of the casein hydrolysate (500 mg/l) presence in the nutrient medium on the gynogenic structures formation frequency in all studied samples of C. pepo, C. maxima and C. moschata was established.
Key words: C. pepo, C. maxima, C. moschata, gynogenesis, doubled haploid
Solovyeva J.A., postgraduate student, Head of the Department Botany, Plant Breeding and Seed Technology. Orcid.org/0000-0001-8517-8729
Monakhos S.G., D.Sci. (Agr.), professor, Head of the Department Botany, Plant Breeding and Seed Technology. E–mail: s.monakhos@rgau-msha.ru. Orcid.org/0000-0001-9404-8862
Russian State Agrarian University – MTAA after K.A. Timiryazev (RSAU-MTAA after K.A. Timiryazev)
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PDF(Rus)
For citing: Solovyeva J.A., Monakhos S.G. Study of the gynogenesis induction factors of the genus Cucurbita L. representatives. Potato and vegetables. 2024. No5. Pp. 36-40. https://doi.org/10.25630/PAV.2024.92.21.002 (In Russ.).
