UDC 635.21:632.4
https://doi.org/10.25630/PAV.2023.23.73.001
Tuchkov I.V., Chudosvetova D.Yu., Tarakanov R.I., Smirnov A.N.
The aim of the study is to evaluate the species composition of Fusarium fungi developing due to late blight, as well as to clarify the sources and localization of Fusarium in potato tubers, taking into account such succession and phytosanitary situation. The research was carried out in the laboratory of the Phytopathology sector of the Department of Plant Protection of the Russian State Agricultural Academy named after K. A. Timiryazev in 2021–2023. The selected tubers of the Red Scarlet and Adretta potato varieties were washed from the soil in tap water. Surface sterilization was carried out according to the appropriate method. To do this, the tubers were immersed in a 2% solution of sodium hypochloride for five minutes, washed three times with sterile distilled water and dried with sterile filter paper. The central part of the sterilized tubers was extracted and cut into cubes with an area of 1 cm2. Further, based on their version of the experiment, fragments of the vascular ring, eyes and periderm were placed on the cube. 80 µl of P. infestans 161 isolate suspension was applied to the samples, from the collection of the Russian State Agricultural Academy named after K.A. Timiryazev (courtesy of the Russian Potato Research Centre in 2014) and incubated in a humid chamber at a temperature of 20–23 °C and moderate lighting for 10 days. Experience options included: 1) sterile cube and periderm, 2) sterile cube and eyes, 3) sterile cube and vascular ring, 4) negative control in the form of a sterile cube. Phytophthora creates penetration points of Fusarium pathogens in potato tubers. Localization of Fusarium fungi in the studied tubers after their defeat by late blight is associated with the periderm of potato tubers. Fusarium species were identified using a combination of morphological, cultural (colony structure, mycelia and conidia) and molecular (PCR and sequencing) characteristics. Molecular identification of the identified fungi of the genus Fusarium revealed a complex of three species: F. oxysporum, F. Proliferatum and F. solani. This fact must be taken into account when producing and storing food and seed potatoes.
Key words: potato late blight, potato tuber dry rot, Phytophthora infestans, Fusarium solani, Fusarium oxysporum, Fusarium proliferatum
Tuchkov I.V., student of the Department of plant protection. E-mail: tuchkov_2002@mail.ru
Chudosvetova D.Yu., student of the Department of plant protection. E-mail: chudosvetova@gmail.com
Tarakanov R.I., post-graduate student of the Department of plant protection. E-mail: tarakanov.rashit@mail.ru
Smirnov A.N., D.Sci. (Biol.), professor of the Department of plant protection. E-mail: asmirnov@rgau-msha.ru
Russian State Agrarian University – Moscow Timiryazev Agricultural Academy
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For citing: Development of Fusarium dry rot after late blight in potato tubers. I.V. Tuchkov, D.Yu. Chudosvetova, R.I. Tarakanov, A.N. Smirnov. Potato and vegetables. 2023. No7. Pp. 32-36. https://doi.org/10.25630/PAV.2023.23.73.001 (In Russ.).
