Creation of resistant potato varieties to damage by the Colorado potato beetle is the main direction of stabilization of potato fields in the long term. The aim of these studies is to improve the methodology for the selection of genotypes with a relatively high resistance to damage by the Colorado potato beetle. A method is proposed for the selection of genotypes with a relatively high resistance to damage by the Colorado potato beetle according to the phenotypic sign of the intensity of the hypersensitivity reaction (HR) of the leaf blade of potato leaves to the clutches of eggs of the Colorado potato beetle. In order to exclude the dependence of the selection screening on the presence of an insect clutch on the leaf blade, an effector that triggers a HR – response to clutches was isolated from the composition of chemical compounds on the chorion surface of the laid eggs. On the basis of two-year field experiments on a large number of cultivars, the presence of a positive correlation dependence of the average degree (coefficient 0.451) between the intensity of necrosis formation in response to the treatment of the leaf blade by the effector that triggers a HR – response and the level of resistance of potato plants to phytophagous was established. The use of the triggering effector HR – the reaction to the egg clutches of the Colorado potato beetle makes it possible to evaluate hybrids in younger nurseries in the absence of a phytophages. The article provides a detailed protocol of an improved methodology for assessing and selecting insect-resistant genotypes using the triggers effector HR – response to egg clutches of the Colorado potato beetle. Stable repeatability of the HR – response of potato leaves to clutches of Colorado potato beetle eggs in the field using the microwave response effector allows visualizing the presence of plant resistance to the pest, greatly facilitating the assessment and selection of resistant genotypes, which ultimately significantly increases the effectiveness of the breeding process.
Key words: Colorado potato beetle, resistance, potato breeding, HR – reaction
Mardanshin I.S., Cand. Sci. (Biol.), head of Laboratory of Potato Breeding and Seed Growing, Subdivision of the Ufa Federal Research Center of the Russian Academy of Sciences. Phone: +7 (905) 181-54-69. E-mail: firstname.lastname@example.org
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For citing: Mardanshin I.S. Improvement of the selection method for potato breeding for resistance to the Colorado potato beetle. Potato and vegetables. No11. Pp. 25-28. https://doi.org/10.25630/PAV.2021.40.15.004 (In Russ.).