The first detection of beet yellows virus (Closterovirus flavibetae) in the Russian Far East

Posted on 13.03.2026 by secretar
UDC 635.112:632.38
https://doi.org/10.25630/PAV.2026.68.39.001

Tolkach V.F., Kakareka N.N., Volkov Yu.G., Shchelkanov M.Yu.

In the process of ecological and virological monitoring of agrophytocenoses in the Primorsky Krai regularly carried out by the Laboratory of Virology of the Federal Scientific Center for the East Asia Terrestrial Biodiversity of the Far Eastern Branch of the Russian Academy of Sciences (FSCEAB) in beetroot plantings (Beta vulgaris ssp. vulgaris var. conditiva) several plants with severe deformation and yellowing of the leaves were identified. A viral isolate Beet/Novitskoe/2018 was obtained from diseased plants, which was deposited as an unclassified strain in the Russian Collection of East Asian Viruses, which operates on the basis of FSCEAB. This paper presents the results of experiments on infection of test plants from the Aizoaceae, Amaranthaceae and Solanaceae families by mechanical rubbing with carborundum, to study the possibility of vector transmission of green peach aphids (Myzus persicae) (the most universal carrier of plant viruses in the Far East), as well as the morphology of virions detected by electron microscopy. Based on the complex of data obtained – the development of a productive viral infection in inoculated test plants, the symptoms manifested in the case of the infection, the level of effectiveness of mechanical and vector transmission, the characteristic size and shape of virions – it was found that Beet/Novitskoe/2018 is a strain of Beet yellow virus (BYV), or Closterovirus flavibetae (Martellivirales: Closteroviridae). This virus is widespread all over the world including the European part of Russia, Central Asia and China, but data on its circulation in the Russian Far East have not yet been available. The discovery of C. flavibetae here requires clarification of the regional phytosanitary control program for vegetable crops.

Key words: beet yellows virus, BYV, Clostero¬virus flavibetae, Closteroviridae, Far East, Primorsky Krai

Tolkach V.F., Cand. Sci. (Biol.), senior research fellow of Laboratory of Virology, Federal Scientific Center for the East Asia Terrestrial Biodiversity of Far Eastern Branch of Russian Academy of Sciences

Kakareka N.N., Cand. Sci. (Biol.), leading research fellow, head of Laboratory of Virology. Federal Scientific Center for the East Asia Terrestrial Biodiversity of Far Eastern Branch of Russian Academy of Sciences

Volkov Yu.G. (author for correspondence), Cand. Sci. (Biol.), senior research fellow of Laboratory of Virology. Federal Scientific Center for the East Asia Terrestrial Biodiversity of Far Eastern Branch of Russian Academy of Sciences

Shchelkanov M.Yu., D. Sci. (Biol.), corresponding member of RAS. Director of G.Р. Somov Institute of Epidemiology and Microbiology, Rospotrebnadzor; Far Eastern Federal University, School of Medicine and Life Sciences, Head of the Department of Biomedicine at the Institute of Medicine and Life Sciences of the Far Eastern Federal University

  1. The formation of phytovirology in the Far East in the context of the development of the virology in Russia. M.Yu. Shchelkanov, N.N. Kakareka, Yu.G. Volkov, V.F. Tolkach Vladivostok: FEFU, 2022. 142 p. DOI: 10.24866/7444-5353-4. EDN: PHVEOU (In Russ.)
  2. Gnutova R.V. Taxonomy of plant viruses in the Far East. Vladivostok: Dalnauka, 2009. 467 p. EDN: QLASLN
  3. Historiography of the “natural focus” term. M.Yu. Shchelkanov, V.A. Aristova, V.M. Chumakov, D.K. Lvov. In: Emerging-reemerging infections in the system of biological safety of Russian Federation. Textbook. Moscow: I.M. Sechenov Moscow Medical Academy Press, 2014. Pp. 21–32. EDN: SAWQMX (In Russ.).
  4. Insects-vectors of viral diseases of potatoes in the Far East. N.N. Kakareka, V.F. Tolkach, M.V. Sapotskiy, Yu.G. Volkov, M.Yu. Shchelkanov. In: A.I. Kurentsov’s Annual Memorial Meetings. 2019. No30. Pp. 191–199. DOI: 10.25221/kurentzov.30.18. EDN: GCVTVW (In Russ.)
  5. Diversity of phytovirus strains in the South of the Russian Far East. N.N. Kakareka, Yu.G. Volkov, V.F. Tolkach, M.Yu. Shchelkanov. South of Russia: ecology, development. 2024. Vol. 19. No3. Pp. 18-34. DOI: 10.18470/1992-1098-2024-3-2. EDN: SVHKXC (In Russ.)
  6. Aphids (Homoptera: Aphididae) – vectors of virus diseases in the Far East. Yu.G. Volkov, N.N. Kakareka, V.F. Tolkach, K.P. Dyakonov, T.V. Moskvina, M.Yu. Shchelkanov. In: A.I. Kurentsov’s Annual Memorial Meetings. 2019. No30. Pp. 211–222. DOI: 10.25221/kurentzov.30.20. EDN: IZRUXZ (In Russ.)
  7. Viral diseases of legumes in the South of the Russian Far East. N.N. Kakareka, Yu.G. Volkov, V.F. Tolkach, T.V. Tabakaeva, Yu.A. Belov, A.A. Muratov, M.Yu. Shchelkanov. South of Russia: Ecology, Development. 2021. Vol. 16. No4. Pp. 71–85. DOI: 10.18470/1992-1098-2021-4-71-85. EDN: ECKPVS (In Russ.)
  8. Viruses of cereal crops and their vectors in the South of the Russian Far East. N.N. Kakareka, Yu.G. Volkov, M.V. Sapotskyi, V.F. Tolkach, M.Yu. Shchelkanov. Agricultural Biology. 2020. Vol. 55. No3. Pp. 439-450. DOI: 10.15389/agrobiology.2020.3.439rus. EDN: VITRAS (In Russ.)
  9. Ecology of rice viruses in the South of the Russian Far East. Yu.G. Volkov, N.N. Kakareka, V.F. Tolkach, A.G. Klykov, M.Yu. Shchelkanov. Rice Research: Open Access. 2024. Vol. 12. No3. id: 409. DOI: 10.4172/2375-4338.1000409
  10. Viruses of Nepovirus genus (Picornavirales, Secoviridae) in the south of the Far East: results of longitudinal monitoring. N.N. Kakareka, Z.N. Kozlovskaya, Yu.G. Volkov, T.I. Pleshakova, M.V. Sapotsky, M.Yu. Shchelkanov. South of Russia: Ecology, Development. 2017. No4. Pp. 105–119. DOI: 10.18470/1992-1098-2017-4-105-119. EDN: ZXREFF (In Russ.)
  11. Cucumber mosaic virus among ornamental crops in the Russian Far East. V.F. Tolkach, Yu.G. Volkov, N.N. Kakareka, M.R. Aliev, M.Yu. Shchelkanov. South of Russia: Ecology, Development. 2023. Vol. 18. No4. Pp. 91–103. DOI: 10.18470/1992-1098-2023-4-91-103. EDN: HTGNHO (In Russ.)
  12. Viruses and their vectors of the vegetable cultures of the Russia Far East / V.F. Tolkach, N.N. Kakareka, Yu.G. Volkov, M.Yu. Shchelkanov. In: A.I. Kurentsov’s Annual Memorial Meetings. 2019. No30. Pp. 200–210. DOI: 10.25221/kurentzov.30.19. EDN: BMOVTJ (In Russ.)
  13. Virus diseases of vegetable and melon crops in the south of the Far East. V.F. Tolkach, N.N. Kakareka, Yu.G. Volkov, Z.N. Kozlovskaya, M.V. Sapotsky, T.I. Pleshakova, K.P. Dyakonov, M.Yu. Shchelkanov. South of Russia: ecology, development. 2019. Vol. 14. No4. P. 121–133. DOI: 10.18470/1992-1098-2019-4-121-133. EDN: HSZHQU (In Russ.)
  14. Organization of Russian State Collection of Viruses from Eastern Asia on the base of Far Eastern Branch of Russian Academy of Sciences. M.Yu. Shchelkanov, Yu.G. Volkov, N.N. Kakareka, Z.N. Kozlovskaya, M.V. Sapotsky, V.F. Tolkach, T.I. Pleshakova, A.V. Gapeka, I.V. Galkina. In: Proceedings of International readings «Maritime Sunrises 2017» (Vladivostok, Russia; 20-22 April, 2017). Vladivostok: FEFU Publ., 2017. Pp. 466–470. EDN: WMDPZA (In Russ.)
  15. Arthropod-vectors of phytoviruses in the Russian Far East: a review. Yu.G. Volkov, N.N. Kakareka, V.F. Tolkach, M.Yu. Shchelkanov. Far Eastern Entomologist. 2024. No510. Pp. 23-32. DOI: 10.25221/fee.510.3. EDN: ICZYCV
  16. Razvyazkina G.M., Polyakova G.P. Electron microscopic study of the winter wheat mosaic virus in the cicada vector Psammotettix striatus L. Reports of the USSR Academy of Sciences. 1970. Vol. 193. No5. Pp. 1171–1173. (In Russ.)
  17. Sapotsky M.V., Kakareka N.N., Polyakova A.M. Diagnostics of phytopathogenic viruses by immunoprecipitation followed by gel electrophoresis. Agriculture Biology. 2001. No5. Pp. 113–116. (In Russ.)
  18. Newly distinguished cell punctures associated with transmission of the semipersistent phloem-limited beet yellows virus. J. Jimenez, W.F. Tjallingii, A. Moreno, A. Fereres. Journal of Virology. 2018. Vol. 92. No21. id: e01076-18. DOI: 10.1128/JVI.01076-18. EDN: RWJFWD
  19. Bottom-up regulation of a tritrophic system by Beet yellows virus infection: consequences for aphid-parasitoid foraging behaviour and development. L. Albittar, M. Ismail, G. Lohaus, A. Ameline, B. Visser, C. Bragard, T. Hance. Oecologia. 2019. Vol. 191. No1. Pp. 113-125. DOI: 10.1007/s00442-019-04467-0. EDN: COBRET
  20. Agranovsky A.A. Closteroviruses: Molecular biology, evolution and interactions with cells // In: Plant Viruses: Evolution and Management. 2016. Pp. 231–252. DOI: 10.1007/978-981-10-1406-2_14. EDN: YVOOUL
  21. Bar-Joseph M., Hull R. Purification and partial characterization of sugar beet yellows virus. Virology. 1974. Vol. 62. No2. Pp. 552-562. DOI: 10.1016/0042-6822(74)90415-2
  22. Kassanis B., Carpenter J.M., White R.F., Woods R.D. Purification and some properties of beet yellows virus. Virology. 1977. Vol. 77. No1. Pp. 95–100. DOI: 10.1016/0042-6822(77)90409-3.
  23. Harrington R., Dewar A.M., George B. Forecasting the incidence of virus yellows in sugar beet in England. Ann. Appl. Biol. 1989. Vol. 114. Pp. 459–469.
  24. De Koeijer K.J., van der Werf W. Effects of beet yellows virus and beet mild yellowing virus on leaf area dynamics of sugar beet (Beta vulgaris L.). Field Crops Research. 1999. Vol. 61. No2. Pp. 163-177. DOI: 10.1016/S0378-4290(98)00155-5. EDN: ACOGBB
  25. New insights into virus yellows distribution in Europe and effects of beet yellows virus, beet mild yellowing virus, and beet chlorosis virus on sugar beet yield following field inoculation / R. Hossain, W. Menzel, C. Lachmann, M. Varrelmann. Plant Pathology. 2020. Vol. 70. No3. Pp. 584-593. DOI: 10.1111/ppa.13306. EDN: LFTKLW
  26. Shurovenkov Yu.B. Methodological guidelines for the identification, accounting and prognosis of the development of sugar beet diseases and signaling the timing of their control. Moscow, 1984. 35 p. (In Russ.)
  27. Rogov V.V., Bobkova A.F., Karasev A.V. Sugar beet jaundice virus: isolation, purification and diagnosis by ELISA. Agricultural Biology. 1990. No1. Pp. 181–186. (In Russ.)
  28. Nucleotide sequence of the 3'-terminal half of beet yellows closterovirus RNA genome: unique arrangement of eight virus genes / A.A. Agranovsky, V.P. Boyko, A.V. Karasev, N.A. Lunina, E.V. Koonin, V.V. Dolja. Journal of General Virology. 1991. Vol. 72. No1. Pp. 15–23. DOI: 10.1099/0022-1317-72-1-15
  29. Stognienko O.I., Stognienko E.S. Field resistance of F1 hybrids of sugar beet to the most harmful diseases in the conditions of the Central Chernozem region. Agrarian Science. 2019. NoS2. Pp. 75–78. DOI 10.32634/0869-8155-2019-326-2-75-78. EDN PKELFS (In Russ.)
  30. Gerami N.M., Mehrvar M., Zakiaghl M. Identification and molecular analysis of beet yellows virus (BYV) in Khorasan Razavi Province. Iranian Plant Protection Research. 2015. Vol. 29. Pp. 55-59. DOI: 10.22067/jpp.v29i1.28056
  31. Discovery of a Closterovirus infecting jujube plants grown at Aksu Area in Xinjiang of China. Q. Lu, G. Wang, Z. Yang, Y. Wang, B. Yang, J. Bai, N. Hong. Viruses. 2023. Vol. 15. No2. id: 267. DOI: 10.3390/v15020267. EDN: UOCLJI

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For citing: The first detection of beet yellows virus (Closterovirus flavibetae) in the Russian Far East. V.F. Tolkach, N.N. Kakareka, Yu.G. Volkov, M.Yu. Shchelkanov. Potato and vegetables. 2026. No1. Pp. 20-24. https://doi.org/10.25630/PAV.2026.68.39.001 (In Russ.).

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