Development of the HybProb molecular marker for the Tsw allele for marker-assisted selection of sweet pepper Capsicum spp. on the real-time PCR platform

Posted on 07.11.2024 by admin
UDC 635.649:631.527
https://doi.org/10.25630/PAV.2024.96.52.001

Budylin M.V., Verba V.M.

One of the conditions for creating a modern high-tech hybrid of sweet pepper is the presence of resistance to the Tomato spotted wilt virus (PMMoV). This virus belongs to the genus Orthotospovirus and leads to large losses of marketable fruits around the world. To date, the only universal source of sweet pepper resistance to some of TSWV pathotypes (pathotype P0) strains is the Tsw gene allele, which was found in Capsicum chinense L. (PI152225 и PI159236). Plants which contain Tsw allele show field resistance supported by hypersensitive reaction mechanism. Many other pathotypes (common named as pathotype P1), which break Tsw-resistance, exists, but Tsw allele is still used in the breeding programs the wide world over. However, there are still no effective molecular diagnostic tools for conducting breeding programs for the introduction of the Tsw allele into the genetic background of parental lines of sweet pepper. Here we report on the development of an accurate and cost-effective molecular marker for marker-assisted selection (MAS) sweet pepper selection based on hybridization probes and a real-time PCR platform. This diagnostic system has been validated on reference germplasm accessions from international genetic banks. The F195 marker's validity has been confirmed by the independent laboratory AgBiotech (USA). Also, using this molecular marker, screening of breeding material of different generations was conducted, stable lines were identified and first-generation hybrids were obtained on their basis, which were tested in production conditions.

Key words: Capsicum, Tomato spotted wilt orthotospovirus, gene resistance, Tsw , SNP, Real-Time PCR.

Budylin M.V., Cand. Sci. (Biol.), deputy director for molecular diagnostics and biotechnology. E-mail: bmw@gavrish.ru

Verba V.M., Cand. Sci. (Agr.), senior research fellow. E-mail: verba@gavrish.ru, verbavm@mail.ru

LLC “Research Institute of Vegetable Breeding” (NIISOK LLC).
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PDF(Rus)

For citing: Budylin M.V., Verba V.M. Development of the HybProb molecular marker for the Tsw allele for marker-assisted selection of sweet pepper Capsicum spp. on the real-time PCR platform. Potato and vegetables. 2024. No7. Pp. 32-36. https://doi.org/10.25630/PAV.2024.96.52.001 (In Russ.).

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