UDC 635.63: 631.527.541: 632.3
https://doi.org/10.25630/PAV.2024.90.27.003
Teplyakova D.D., Shevkunov V.N.
Today, the problem of crop loss due to damage to cucumber hybrids by the cucumber green mottled mosaic virus (CGMV) remains very urgent. The most effective method of prevention is the selection of resistant hybrids. When creating them during the breeding process, the continuous adaptability of the virus must be taken into account; therefore, it is necessary to regularly evaluate the breeding material for resistance to diseases and pests. We were tasked with optimizing the breeding process in our conditions by selecting a method for rapid assessment of the resistance and susceptibility of cucumber plants to IBD, on an artificially created infectious background, to create modern stable and tolerant hybrids.of cucumber plants to CGMV, against an artificially created infectious background. The experience was laid down in three repetitions. 8 samples were studied: parent lines and two standards susceptible – F1 Kibria and stable – F1 Verdon. The parent lines were selected according to the degree of resistance based on the total results of the assessment carried out in greenhouses in different turns on a natural infectious background. Two stable, tolerant or weakly receptive and unstable lines were selected. The material was sown in cassettes for seedlings, with peat soil. In each plot there were 20 plants, of which 16 were infected, and four plants were left untreated as a control. Having analyzed four methods of infecting plants (1 – dripped into the growing point, 2 – rubbed the inoculum into one cotyledon, 3 – rubbed into two cotyledons and 4 – buried into the growing point and rubbed into two cotyledons) we came to the conclusion that the highest level of effectiveness showed the second method. In the third and fourth methods, the effectiveness decreases, that is, with an increase in the infectious load, objectivity is lost, since resistant samples show signs of CGMV.The Model 5629/22 was chosen as a promising, productive, modern tolerant hybrid to the CGMV.
Key words: cucumber, CGMV, artificially created infectious background.
Teplyakova D.D., research fellow. E-mail: ira.kalinovskaya@mail.ru. Phone: +7 (928) 247-64-94.
Shevkunov V.N., deputy director for science. E-mail: vshevkunov@mail.ru. Phone: +7 (928) 282-82-21.
LLC «Research Institute of Vegetable Breeding»
- Akhatov A.K., Akhatov E.A. The most harmful diseases of vegetable crops in modern greenhouse plants. Gavrish. 2014. No3. Pp. 16–23 (In Russ.).
- Istomina E.A., Korostyleva T.V., Konopkin A.A. et al. Protection of cucumber from viral infection. Gavrish. 2016. No6. Pp. 56–61 (In Russ.).
- Plotnikov K.O., Pashkovsky S.E. Study of the dependence of the viral load and the manifesting symptoms of the cucumber green mottled mosaic virus. Newest directions in the development of agricultural science in the works of young scientists: collection of materials of the VII international scientific and practical conference. Novosibirsk. 2019. Pp. 100–103 (In Russ.).
- Evaluation and selection of cucumber for resistance to green mottled mosaic virus: guidelines: O.V. Yurina, N.V. Nastenko, N.P. Cheremushkina, K.A. Mozhaeva Moscow. Agropromizdat. 1990. 17 p. (In Russ.).
- Istomina E. A., Andreeva E. N., Slavokhotova A. A. et al. Vaccination is an effective way to protect plants from cucumber green mottled mosaic virus. Gavrish. 2014. No4. Pp. 41–45 (In Russ.).
- Gordeeva E.I., Kryukova A.V., Kurbatova Z.I. Plant immunity: textbook. Velikie Luki. 2011. Pp. 101–118 (In Russ.).
- Disease resistant cucumber plants. Patent number: RU2418405C2 [Web resource]. URL: https://patents.google.com/patent/RU2418405C2/ru/ Access date: 17.10.2024/
- Grigorovskaya P.I. Establishing the infectivity of a disease. [Web resource]. URL: https://www.pesticidy.ru/dictionary/Establishing_the_infectivity_of_the_disease. Access date: 12.10.2022 (In Russ.).
- Grinko N.N., Green speckled mosaic of cucumbers in protected ground, Bulletin of the Russian Academy of Agricultural Sciences. 2005. No1. Pp. 53–55 (In Russ.).
- Akhatov A.K. The world of cucumber through the eyes of a plant pathologist. Moscow. Society if Scientific publications KMK. 2020. Pp. 132–135 p. (In Russ.).
For citing: Teplyakova D.D., Shevkunov V.N. Selection of an express method for infecting cucumber plants to assess the resistance to CGMV. Potato and vegetables. No7. Pp. 37-40. https://doi.org/10.25630/PAV.2024.90.27.003 (In Russ.).
