Molecular genetic analysis of F1 tomato hybrids for resistance to Fusarium wilt

Posted on 06.05.2021 by Саша Корнев
UDC 635.64:631.52:632
https://doi.org/10.25630/PAV.2021.44.34.006

Eroshevskaya A.S., Egorova A.A., Milyukova N.A., Pyrsikov A.S.

One of the most dangerous diseases of tomato is fusarium wilt, caused by phytopathogenic fungus Fusarium oxysporum f. sp. lycopersici. The growing of tomato resistant varieties and hybrids is the most effective method to control this disease. Now plant analysis for alleles of resistance genes is successfully carried out using molecular markers that allow to identify differences in studied samples at DNA level. The aim of the research is a molecular genetic analysis of tomato F1 hybrids selected by the Poisk AgroFirm for resistance to fusarium wilt (gene I2). As an object of research, 17 hybrids of tomato F1 of different product groups (large-fruited, brush, cocktail, cherry) were taken. The analysis was carried out in the laboratory of marker and genomic plant breeding of FSBSI VNIISB in 2019. The functional marker I-2 with primers I-2/5F (CAAGGAACTGCGTCTGTCTG) and I-2/5R (ATGAGCAATTTGTGGCCAGT) was used to identify I2 gene alleles. The PCR was carried out in the Termal Cycler Bio-Rad T 100 amplifier, the results were visualized by electrophoresis in a 1.7% agarose gel with 1x TAE buffer and were analyzed using the Gel Doc 2000 system. The fragments 633 bp (I-2 allele) and 566 bp (I-2C allele) in investigated tomato hybrids indicate their resistance to this disease. Among 17 hybrids 16 are resistant to fusarium wilt, 4 hybrids from them are dominant homozygotes for I2 gene (I-2 alleles). Hybrid F1 835/19 has both I-2 and I-2C alleles. To test the I2 gene effectiveness it is planned to assess tomato F1 hybrids by artificial inoculation in seedling phase (Fusarium oxysporum f. sp. lycopersici races 1 and 2). Dominant homozygotes for I2 gene will be used in breeding programs for creating donor lines of resistance to fusarium wilt if the results of marker analysis are confirmed.

Key words: tomato, hybrid, fusarium wilt, molecular genetic analysis, resistance, donor

Eroshevskaya A.S., post-graduate student, junior research fellow, ARRIVG – branch of FSBSI FSVC. E-mail: eroshnast@yandex

Egorova A.A., Cand. Sci. (Agr.), senior research fellow, ARRIVG – branch of FSBSI FSVC. E-mail: edvaaed@rambler.ru

Milyukova N.A., Cand. Sci. (Biol.), senior research fellow of laboratory of marker-assisted and genomic plant breeding, FSBSI All-Russian Research Institute of Agricultural Biotechnology (ARRIAB), associate professor of the Department of Genetics, Selection and Seed Production, Russian State Agrarian University – Moscow Timiryazev Agricultural Academy (RSAU – MTAA). E-mail: milyukovan@gmail.com

Pyrsikov A.S., Cand. Sci. (Agr.), research fellow of laboratory of marker-assisted and genomic plant breeding, FSBSI ARRIAB. E-mail: andrey.pyrsikov@yandex.ru

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PDF(Rus)

For citing: Molecular genetic analysis of F1 tomato hybrids for resistance to Fusarium wilt. A.S. Eroshevskaya, A.A. Egorova, N.A. Milyukova, A.S. Pyrsikov. Potato and vegetables. 2021. No5. Pp. 37-40. https://doi.org/10.25630/PAV.2021.44.34.006 (In Russ.).

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