Sinicyna A.A., Vishnyakova A.V., Monakhos S.G.
The technology of doubled haploids production in isolated microspore culture of Brassica crops is used to accelerate and cheapen the process of creating parental lines of F1-hybrids in comparison with traditional breeding. Data on the relative yield of doubled haploids with using the standard protocol of isolated microspore culture are of practical interest for biotechnologists and breeders. The aim of the study is a comparative assessment of the yield of doubled haploids obtained in isolated microspore culture of white cabbage (Brassica oleracea var. capitata L.) and rapeseed (Brassica napus L.). Doubled haploids of white cabbage and rapeseed were obtained in 2021–2022 at the Timofeev Breeding Station and the Laboratory of Genetics, Breeding and Biotechnology of Vegetable Crops, RSAU – MTAA. The following were used as microspore donor plants: 5 samples of white cabbage, represented by commercial hybrids F1 Kaptur, F1 Larsia, selection samples 101f3×15dg1, MF4MC, Ges2×15) 4–4 from the collection of «Timofeev Breeding Station»; and 3 commercial rapeseed hybrids F1 Jazz, F1 Factor, F1 Majong. It is shown that the frequency of embryogenesis of white cabbage accessions can be high and comparable to the frequency of rapeseed embryogenesis. The frequency of germination/regeneration of seedlings of the studied accessions of white cabbage and rapeseed was high and averaged 70%: in white cabbage it varied from 63.3% to 75.1%, in rapeseed – from 65.2% to 73.9%. When assessing the level of ploidy of the obtained plants, it was found that the frequency of spontaneous diploidization in white cabbage is higher (90–100%) than in rapeseed (21.4–32%). As a result, out of 83 rapeseed regenerants, only 21 were doubled haploids, and in white cabbage out of 87 regenerative plants, 83 were doubled haploids.
Key words: embryogenesis, isolated microspore culture, regeneration, white cabbage, rapeseed, doubled haploids, spontaneous doubling
Sinitsyna A.A., postgraduate student of the Department of Botany, Breeding and Seed Production of Garden Plants of the Russian State Agrarian University–Moscow Timiryazev Agricultural Academy. E-mail: firstname.lastname@example.org. Phone: (925) 176-67-49; orcid.org/0000-0002-9470-3676
Vishnyakova A.V., Cand. Sci (Agr.), associate professor of the Department of Botany, Breeding and Seed Production of Garden Plants of the Russian State Agrarian University–Moscow Timiryazev Agricultural Academy. E-mail: email@example.com. Phone: (499) 976-41-71; orcid.org/0000-0002-9160-1164
Monakhos S.G., D. Sci. (Agr.). Head of the Department of Botany, Breeding and Seed Production of Garden Plants, the Russian State Agrarian University–Moscow Timiryazev Agricultural Academy. E-mail: firstname.lastname@example.org. Tel.: (499) 976-41-71; orcid.org/0000-0001-9404-8862
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For citing: Sinicyna A.A., Vishnyakova A.V., Monakhos S.G. Comparative assessment of the yield of doubled haploids of Brassica oleracea var. capitata L. and Brassica napus L. in isolated microspore culture. Potato and vegetables. 2022. No4. Pp. 37-40. https://doi.org/10.25630/PAV.2022.29.31.008 (In Russ.).